DiTag? pH-sensitive IgG labeling reagents offer a convenient way to measure antibody internalization. These reagents use a pH-sensitive fluorescent dye conjugated Fc binding protein, which forms a complex with IgG antibodies. Upon internalization, the acidic environment enhances the fluorescence signal, indicating internalization activity. By measuring fluorescence intensity, researchers can assess internalization efficiency and gain insights into antibody uptake mechanisms, helping optimize antibody-based therapies and drug delivery systems.

Comparison of internalization efficiency between AME100002 and a competitor reagent on GPRC5D-positive cells (K562-GPRC5D stable expression cell line). AME100002 requires less reagent and shorter incubation time while demonstrating higher sensitivity. It is the most cost-effective pH-sensitive fluorescence IgG labeling reagent available. This pH-sensitive IgG labeling reagent is compatible with human IgG1, IgG2, IgG3, IgG4, rabbit IgG, and mouse IgG subtypes (IgG1, IgG2a, IgG2b, IgG3).

Payload-conjugated IgG labeling reagents use a payload-conjugated Fc binding protein to label various IgG antibodies. Once the IgG-payload complex is added to the cells, the cell inhibition rate for each sample is determined using the CCK8 method. This approach mimics the real ADC mechanism, where the antibody delivers the payload into the cells, resulting in cell death. By measuring the cell inhibition, researchers can evaluate the effectiveness of the candidate mAbs and gain insights into the potential of ADCs for therapeutic applications.

Cell inhibition rate of HeLa cells detected using the CCK8 method. B7H3 ADC BMK was mixed with DiTag? MMAE IgG labeling reagent (Cat. No. AME100003) to form a complex, which was applied to HeLa cells for the inhibition test. The results were compared to the control, where Isotype Control IgG was mixed with AME100003 and applied to the cells. The IC50 of B7H3 ADC BMK is 66 ng/ml.

Accelerated stability test of AME100003. Following lyophilization, samples were stored at -20°C (black), 37°C for 1 day (red), 37°C for 2 days (blue), and 37°C for 3 days (green), separately. Upon reconstitution, the cell inhibition rate of each sample was determined using the CCK8 method. The data indicate excellent stability for all samples.