膜蛋白案例展示

DIMA生物科技是一家專注于蛋白質研究的公司，他們提供了一系列由哺乳動物細胞表達系統(tǒng)制備的蛋白質產(chǎn)品。這些產(chǎn)品經(jīng)過嚴格的功能驗證，目錄中包含了1000+現(xiàn)貨蛋白質，以滿足全球科學家和研究人員的需求。為了確保產(chǎn)品的質量和可靠性，DIMA生物科技采用凍干脫水方式進行寄送，這種保存方法可以保證長期儲存并在重新溶解后保持蛋白質的穩(wěn)定性和活性。因此，科學家可以對DIMA生物科技的蛋白質產(chǎn)品充滿信心，并放心地在實驗中使用，因為它們始終品質如一。

Extracellular domain (ECD)
Full-length Membrane Proteins

對于單次穿膜蛋白來說，DIMA可提供功能活性和高穩(wěn)定性的胞外區(qū)重組蛋白（ECD蛋白）。這些蛋白經(jīng)過廣泛測試和驗證，以確保其功能完整性，使它們非常適用于藥物研究和開發(fā)的各種應用場景。

High Purity,? High Bioactivity

Protein Purity

Figure 1. Human SIRPa, hFc-His Tag (A) and CD47, mFc-His Tag (B) on SDS-PAGE under reducing condition.

Receptor-ligand interaction

Figure 2. Human CD47 protein (PME100008) can bind its native ligand Human SIRPα protein (PME100009) in a linear range of 3.3-26.37 ng/ml.

Biosimilar interaction

Figure 3. Human CD47 protein (PME100008) can bind Anti-CD47 mAb (BME100001) in a linear range of 0.061-1.606 ng/ml.

Freeze-thaw stability testing

Protein Purity

Lane 1: Human SIRPα protein without freeze-thaw treatment; Lane 2: Human SIRPα protein after one freeze-thaw cycle; Lane 3: Human SIRPα protein after three freeze-thaw cycles; Lane 4: Human SIRPα protein after five freeze-thaw cycles.

Receptor-ligand interaction

A: Human SIRPα without freeze-thaw treatment; B: Human SIRPα protein after one freeze-thaw cycle; C: Human SIRPα protein after three freeze-thaw cycles; D: Human SIRPα protein after five freeze-thaw cycles.

Storage Stability: Shelf Life Testing

ACE2-hFc, 37℃, 1, 2, 3 days storage stability

Lane 1: Human ACE2 protein after storage at -20℃; Lane 2: Human ACE2 protein after storage at 37℃ after 24h; Lane 3: Human ACE2 protein after storage at 37℃ after 48h; Lane 4: Human ACE2 protein after storage at 37℃ after 72h.

S-RBD protein (PME100497) can bind Human ACE2 protein (?PME100073) (Stored at -20℃ or 37℃ for 24h, 48h and 72h) in a linear range of 0.488-49.83 ng/ml.

CCR8-hFc, 37℃, 1, 2, 3 days storage stability

Lane 1: Human CCR8 protein (PME101091) after storage at -20℃; Lane 2: Human CCR8 protein after storage at 37℃ after 24h; Lane 3: Human CCR8 protein after storage at 37℃ after 48h; Lane 4: Human CCR8 protein after storage at 37℃ after 72h.

Human CCR8 protein (PME101091) ( (Stored at -20℃ or 37℃ for 24h, 48h and 72h) ) can bind Anti-CCR8 Neutralizing antibody (BME100063) in a linear range of 3.2-80 ng/ml.